Good Water-Soluble Mes Buffer pH Range for Cell Culture CAS 4432-31-9

• CAS-Nr.: 4432-31-9
• Formel: C6h13no4s
• EINECS: 224-632-3
• Klassifikation: Biochemische Reagenzien
• Klasse: AR
• Spezifische Nutzungs: Für biologische Zweck, Für Gewebe Medium Zweck, Technische Qualität, Praktische Nutzung, Pro Analyse
• Modell Nr.: Mes Buffer
• Inhalt: Standard
• Verwendung: Laborreagenzien, Diagnostische Reagenzien, Teaching Reagenzien
• Quelle: Trockenpulver
• Habit Appellation: Mes Buffer
• Anwendung: Wissenschaftliche Forschung, Gesundheit
• Immobilien: Biochemischen Reagenzien
• Farbe: Weiß
• Lagerbedingungen: Zimmertemperatur
• Formular: Pulver
• Wasserlöslichkeit: Löslich
• Reinheit: Über 99 %
• Molekulargewicht: 195.24
• Verwenden Sie bis: Biologischer Puffer
• oem-Verpackung: ja
• Hersteller: hubei xindesheng
• Transportpaket: Carton
• Spezifikation: 500g/bottle
• Warenzeichen: DeSheng
• Herkunft: Ezhou City, Hubei Province, China
• Produktionskapazität: 5 Tons Per Month

Produktbeschreibung

Packaging Specification : Plastic bottles, 500g/bottle

Basic Information
 

Abbreviation	MOPS	CAS No	1132-61-2
Molecular formula	C7H15NO4S	Molecular weight	209.27
Purity	≥99%	Appearance	White crystal powder
pKa	7.2	pH range	6.5-7.9

 

    


Advantages of MES buffer

The pka value is in the neutral range; The Pka value is not affected by temperature and changes; The powder has good water solubility and is not easily soluble in other solvents; Difficult to chelate with salt ions; Good use effect and strong stability; There is no significant light absorption in the ultraviolet and visible light bands; Easy to synthesize

Preparation method of MES buffer:

1. Prepare 800 mL of dH2O in a suitable container.

2. Add 97.62 grams of MES free acid to the dH2O solution.

3. The initial pH value of 0.5M MES solution is 3.23. For 1 L of 0.5M MES, 13.6 ml of 10N NaOH is required to adjust the pH to 6.0;

4. Add dH2O until the volume is 1L.

Storage: MES solution can be stored for several months at 2-8 ºC. It is recommended to use a 0.2 micron filter membrane for filtration and sterilization. (Substances containing sulfate ions are not easily sterilized at high temperatures) After high-temperature sterilization, MES will show a light yellow color. It is not clear what the light yellow degradation product is, but the pH will not change significantly.



Application of MOPSO

As anion buffer, MES buffer is suitable for cation exchange chromatography and hydroxyapatite chromatography; It can be used for gel filtration chromatography; Can be used for cellulose phosphate column chromatography; Can be used for hydrophobic substance interaction chromatography; Can be used for SDS-PAGE; Can be used for fluorescence microscopy; Suitable as a non coordinating buffer in metal ion solutions; Due to the fact that MES buffer does not form free radicals, it is suitable for the study of redox processes; Because it will not be metabolized by bacteria and eukaryotic cell, MES buffer can be used to prepare culture media, such as low concentration plant cell culture media and mammalian cell culture media; Suitable for most toxin research; Used for in vitro dissolution test of liposomes; The interaction between MES buffer and the peptide skeleton of bovine serum albumin can enhance the stability of the protein
 

 
Product Advantages

Purity ≥ 99%, good water solubility, stable process, and can ensure that the product appearance is pure white crystal powder.



 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!